The study, “Ultrasensitive Sniff-Cam for Biofluorometric-Imaging of Breath Ethanol Caused by Metabolism of Intestinal Flora,” appeared in the journal Analytical Chemistry.
The systems used to date to measure VOCs have been bulky and expensive, and require trained professionals to operate them. In turn, smaller and more cost-effective options have shown lack of selectivity.
More recent techniques have coupled biochemical analysis with imaging. These so-called bio-sniffers — fiber-optic biochemical gas sensors — have taken advantage of enzymes dependent on a cellular molecule called nicotinamide adenine dinucleotide (NAD) to achieve high selectivity. These approaches can effectively measure ethanol, a product of the microbiome — the community of bacteria, viruses, and fungi that live within our bodies — and be an indicator of glucose (sugar) levels.
A gas visualization system was then developed to measure ethanol release by the skin after alcohol consumption. However, its ability to detect ethanol levels was inferior to that of bio-sniffers.
In the new method, an enzyme mesh creates a reaction between ethanol and oxidized NAD (NAD+), producing fluorescence, which is then recorded with the camera.
The system was then tested in 10 healthy men (ages 24–28) who had not consumed alcohol, smoked, or taken a medication within 72 hours of the experiment.
The measurements, performed during a four-hour period of fasting, revealed that the sniff-cam was able to detect even minuscule amounts of ethanol in the breath. Of note, the average concentration of ethanol was similar to that reported in a prior study, conducted over a two-hour period during consumption of a sweet drink or meal. However, unlike the sniff-cam, this previous work used a non-portable system.
They also noted that the new system may be smaller if adopting a more compact UV-LED and image sensor. “Therefore, the sniff-cam system may be used in smartphones in the future to allow people to simply monitor their own health status.”